University of California, Berkeley 의 Luke P. Lee 교수님의 세미나가 있습니다.
관심있으신 분들의 많은 참여 바랍니다.
강사: Professor. Luke P. Lee
(Department of Bioengineering, University of California, Berkeley)
주제: Satellite Nanoscopes for the Exploration of Living Cells
일시: 2010년 2월 8일(월) 11:00
장소: 서울대학교 공과대학 301동 612호
요약: Our understanding of biological systems is increasingly dependent on our ability to visualize and measure biomolecules and biological events with high spatial and temporal resolution in a living cell. Current fluorescence and confocal microscopy requires fluorescent labeling steps. The electron microscope (EM) can resolve subcellular structures without labeling, but EM imaging damages living cells. Moreover, fluorescence and EM microscopy cannot provide spectroscopic information (i.e. chemical fingerprints). Innovative nanoplasmonic local light sources and Plasmonic Resonance Energy Transfer (PRET) nanospectroscopy offer striking advantages over traditional molecular imaging techniques: stability, biocompatibility, selectivity, and spectroscopic imaging capability. Using intracellular nanoplasmonic probes, we obtain snapshots of what we, metaphorically speaking, refer to as the Cellular Galaxy. In essence, the nanoplasmonic probes are functioning as “nano-satellites” to capture the molecular imaging inside of cells and to communicate to outside of cells.
We also have developed Oligonucleotides on a Nanoplasmonic Carrier Optical Switch (ONCOS) for the remote optical control of gene regulation and protein expression as well as therapeutic applications. The Optogenetic ONCOS allows on-demand gene silencing with nanometer-scale spatial resolution and localized temperature controls in living cells. The ONCOS and PRET are being applied for molecular/cellular diagnostics, therapeutic applications, and experimental system biology since these nanoplasmonic biophotonic devices will provide us precise spatial and temporal controls of gene interferences and spectroscopic information of living cellular mechanism. Bionanophotonic molecular ruler is also accomplished to measure the dynamics of DNA and protein interactions and understanding cellular dynamics. In-vivo Surface Enhanced Raman Spectroscopy (SERS) probes, in-vitro integrated nanofluidic SERS, and optofluidic ICs are developed for label-free molecular diagnostics and drug discovery. All these nanosatellites can impact on precision biology, bionanoscience, optogenetics, and nanomedicine.
Prof. Luke P. Lee is Lloyd Distinguished Professor of Bioengineering at UC Berkeley. He is also co-director of Berkeley Sensor &Actuator Center. His current research interests are cell reprogramming, bionanoscience, molecular diagnostics, personalized medicine, and Bioinspired Photonics-Optofluidics-Electronics Technology and Science (BioPOETS). Prof. Lee has authored and co-authored over 220 papers on nanobiophotonics, single cell analysis, quantitative microfluidic cell biology, BioMEMS, SERS, SQUIDs, and nanoplasmonic biosensors for label-free biomolecule detection.
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